Interspecific Hybridization between Aspergillus nidulans and Aspergillus rugulosus by Fusion of Somatic Protoplasts
نویسندگان
چکیده
منابع مشابه
Isolation of nuclei from Aspergillus nidulans protoplasts.
Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifugation was applied. The resulting nuclei were of good purity and morphological s...
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ETEROZYGOUS diploid strains of Aspergillus nidulans spontaneously proHduce segregant clones belonging to three classes: diploid segregants which have undergone mitotic crossing over; haploid segregants with random redistribution of whole chromosomes (PONTECORVO, TARR GLOOR and FORBES 1954) ; and "nondisjunctional" segregants with all the markers on both branches of one chromosome in the homozyg...
متن کاملAspergillus nidulans
Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veAl) , which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veAl that restore the light-dependent conidiation phenotype. Alle...
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Aspergillus nidulans strain G191 was transformed to hygromycin resistance using plasmid pDH25, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the A. nidulans trpC gene. Southern hybridizations of transformants revealed multiple, integrated copies of the vector. A pleiotropic effect conferring increased hygromycin B sensitivity was found to ...
متن کاملMitosis in Aspergillus nidulans.
Mitosis in growing hyphae of haploid and diploid Aspergillus nidulans has been studied with the light microscope in Helly-fixed preparations. At the periphery of the nucleus a sharply defined granule is invariably found which has strong affinity for acid fuchsin. Mitosis begins with the duplication of this granule and the development inside the nucleus of a gradually lengthening fibre between t...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1977
ISSN: 0022-1287
DOI: 10.1099/00221287-102-2-255